EPG PROGRAM

EPG Workshop (HPIS 2017 associated)Image EPG Program

          Madrid – 2016, June 8-10 2017

Freddy Tjallingii, Greg Walker, Alberto Fereres & Elisa Garzo


PROGRAM

Thursday 8th June:

08:45 Registration

09:15 EPG recording, an introduction

09:45 The EPG system, electronic designs & backgrounds I

10:30      coffee break

11:00  Computerized EPG recording (data acquisition)

11:45  Transmission of plant pathogens (Alberto Fereres)

12:30  4 participant presentations, 10 min. each

13:10 lunch break

 

14:30  Afternoon ‘Hands-on’

(16:00 break)

18:30 Plenary discussion

19:00  End

 

Friday 9th June:

09:00   The EPG system, electronic designs & backgrounds II

09:45   Waveform analysis & EPG variables

10:30      coffee break

11:00   Data processing & statistics

11:45   Host plant resistance (FT)

12:30   4 participant presentations, 10 min. each1

13:10      lunch break

 

14:30   Afternoon ‘Hands-on’

(16:00    break)

18:30 Plenary discussion

19:00 End

 

Saturday 10th June:

09:00   Other EPG systems

09:45   Designing EPG experiments

10:30      coffee break

11:00   Measuring plant signals (FT)

11:45   4 participant presentations, 10 min. each1

12:30   4 participant presentations, 10 min. each1

13:10      lunch break

 

14:30   Afternoon ‘Hands-on’

(16:00     break)

18:30  Plenary discussion

19:00 End

20:00 Closing, Certificates, Farewell diner

 

AFTERNOON Hands-On PROGRAM

Day 1

     Beginners Program

EPG recording (data acquisition)

DEMO EPG set up:

– Equipment parts

– Noise and Faraday cage

– Amplifier test*

Aphid wiring

– Making an insect electrode (done)

– Proper electrode attachment

– Aphid and plant mounting*

Making a first EPG recording

– PROBE/Stylet+ acquisition software*

– Start: name, comments &

adjustments

– starting*: 1) Giga ON

2) ‘insects ON’

– Optimal voltage adjustment*

– 8 hour EPG recording

 

Day 2

PROBE software practice

File conversion

– Automatic and comment lines

– Manual conversion (separate conversion)

– comment line changes

Analysis software PROBE/Stylet+

– Use of day 1 data files or sample files

– Moving, zooming and .ppt signal pictures

– exporting signal samples

– Making a ‘Book of reference signals’

Retrieving EPG data

– Cursor use and data grid

– Saving the data grid

frequency & smooth tools

More EPG recording

– Special plant voltage adjustments*

– Use of the calibration button

 

Day 3

Data processing

Sytlectomy with EPG

       demonstration by Elisa Garzo

Use of Excel macros

– BAZ & CSIC macros for data processing

“Help desk”:

Installation of hard- & software

– Downloading and installation Stylet+

– Bring in any questions or problems !

 

* Program items that can be extended for more advanced users:

– Special plant voltage adjustments

– Life establishing of R- and emf-components

– Event marks in unused channels

– Use of the calibration button and emf mode probes (innovation)